EFFECT OF THIOTRIAZOLIN ON PATIENTS BLOOD SERUM CYTOKINES CONTENT IN DIFFERENT STAGES OF NON-ALCOHOLIC FATTY LIVER DISEAS

Murenets N.A., Orlovsky V.F.; Fortune Ufomba, Leah Mwandandila, student of 4th course

Sumy State University, Department of Internal Medicine postgraduate education with propedeutics course

 

Aim: To study the inflammatory cytokine (tumor necrosis factor-α (TNF-α)) and anti-inflammatory cytokine (interleukin-4 (IL-4)) content in different stages of non-alcoholic fatty liver disease (NAFLD) against the background of thiotriazolin usage.

Patients and methods: 33 patients with non-alcoholic simple steatosis (NASS) (I group) and 33 patients with non-alcoholic steatohepatitis (NASH) (II group) were examined. The control group consisted of 20 healthy persons without fatty liver infiltration as demonstrated by ultrasound investigation. NALS were identified by the ultrasound investigation, NASH was diagnosed in case of increased serum transaminase level (no more than 4-th rates). Thiatriazolin was prescribed - domestic hepatoprotector with immunomodulatory properties – 2 ml 2,5% intramuscularly during 5 days, then 100 mg (1 tabl.) 3 times daily during 20 days. Patients were grouped according to their sex and age. Serum cytokines levels were evaluated by the immune-enzyme assay with the usage of appropriate kits before and after the treatment. Data were handled statistically.

Results: In patients with NASS and NASH before treatment, comparing with the control group, significant increase of the serum TNF-α levels were found (respectively 49,93±3,82pg/ml; 87,9±5,41pg/ml; 24,2±2,25pg/ml; р<0,05). Serum IL-4 levels were significantly increased only in patients with NASH, compared to the control group (respectively 41,2±4,3pg/ml; 33,1±2,11pg/ml; р<0,05). However, the significantly increased levels of the inflammatory cytokine did not coincide with the increased levels of the anti-inflammatory cytokine. After the treatment with a thiotriazolin serum TNF-α levels significantly decreased in both groups (I group – to 28,9±2,18pg/ml; II group – to  33,36±2,78mg/l; р<0,05), serum IL-4 levels in the  group II of patients approached the indexes of that of the control group.

Conclusions: Cytokine imbalance was observed in patients with NAFLD. Thiotriazolin promotes significant decrease of the TNF-α levels in different stages of NAFLD and normalization of the serum IL-4 levels in patients with NASH. Thus, alignment of the cytokine imbalance has been observed, which plays a key role in the NAFLD pathogenesis.